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KMID : 0613820120220121658
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Journal of Life Science
2012 Volume.22 No. 12 p.1658 ~ p.1664
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Conjugated Linoleic Acid (CLA) Ameliorates Hydrogen Peroxide-Induced Oxidative Stress on Rat Cardiomyoblast H9c2 Cells
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Park Jae-Hong
Moon Yeon-Gyu
Kwon Jung-Min
Cho Yong-Un
Kim Jeong-Ok
Ha Yeong-Lae
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Abstract
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Conjugated linoleic acid (CLA) exhibits several beneficial biological activities including anticarcinogenesis and body-fat reduction. Now, we report that CLA ameliorated the oxidative stress in rat cardiomyoblast cells, H9c2, treated with hydrogen peroxide (H₂O₂). Cells were cultured in DMEM/F-12 media at 37℃ with humidified atmosphere of 5% CO₂. The cells, cultured for 48 hrs, were seeded at a density 3.5×10³ cell/well in a 24 well-plate and incubated for 24 hr. Using these cells, two experiments were performed: the cytotoxicity test of CLA (10, 20, 30, 40, and 50μMs), and the oxidative stress amelioration test of CLA (20 and μMs) against cells treated with H₂O₂ (10 and 50 μMs) for 1 and 2 hrs. CLA enhanced the growth of H9c2 cells at any concentrations of CLA and at any incubation times (up to 6 days), indicating that CLA acts as a growth stimulant. No protective effect of CLA (20 and 50μMs) was seen in cells treated 50μM H₂O₂ for 1 and 2 hr, but these CLA concentrations ameliorated (p<0.05) the adverse effect of 10μM H₂O₂ in cells treated for 1 hr. These CLA concentrations significantly (p<0.05) reduced the proportion of apoptotic cells, relative to control cells. These results suggest that CLA protected H9c2 cells from the oxidative stress of H₂O₂ through the suppression of cell apoptosis and could be a useful compound for the prevention of cardiac diseases caused by oxidative stress.
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KEYWORD
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Conjugated linoleic acid (CLA), hydrogen peroxide (H₂O₂), rat cardiomyoblast cell H9c2, cytotoxicity, apoptosis
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